Detection of endogenous erbB3: Whole cell lysates of EGF stimulated serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab erbB3-5A12 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: MDA-MB 231; lane 2: MDA-MB 468; lane 3: MCF-7; lane 4: T47D; lane 5: SW480; lane 6: SW620
Detection of endogenous erbB3: Whole cell lysates of EGF stimulated serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab erbB3-5A12 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: MDA-MB 231; lane 2: MDA-MB 468; lane 3: MCF-7; lane 4: T47D; lane 5: SW480; lane 6: SW620
ErbB 3 (ERBB3) (Cytopl. Dom.) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 5A12]
AM00055PU-N
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetERBB3
Overview
- SupplierOriGene
- Product NameErbB 3 (ERBB3) (Cytopl. Dom.) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 5A12]
- Delivery Days Customer14
- ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID5A12
- Gene ID2065
- Target nameERBB3
- Target descriptionerb-b2 receptor tyrosine kinase 3
- Target synonymsc-erbB3; c-erbB-3; ErbB-3; erbB3-S; FERLK; HER3; human epidermal growth factor receptor 3; LCCS2; MDA-BF-1; p180-ErbB3; p45-sErbB3; p85-sErbB3; proto-oncogene-like protein c-ErbB-3; receptor tyrosine-protein kinase erbB-3; tyrosine kinase-type cell surface receptor HER3; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3; VSCN1
- HostMouse
- IsotypeIgG1
- Protein IDP21860
- Protein NameReceptor tyrosine-protein kinase erbB-3
- Scientific DescriptionErbB 3 (ERBB3) (Cytopl. Dom.) (incl. pos. control) mouse monoclonal antibody, clone 5A12, Purified
- ReactivityHuman
- UNSPSC12352203