
Detection of endogenous erbB3: Whole cell lysates of EGF stimulated serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab erbB3-5A12 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: MDA-MB 231; lane 2: MDA-MB 468; lane 3: MCF-7; lane 4: T47D; lane 5: SW480; lane 6: SW620
ErbB 3 (ERBB3) (Cytopl. Dom.) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 5A12]
AM00055PU-N
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetERBB3
Overview
- SupplierOriGene
- Product NameErbB 3 (ERBB3) (Cytopl. Dom.) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 5A12]
- Delivery Days Customer14
- ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID5A12
- Gene ID2065
- Target nameERBB3
- Target descriptionerb-b2 receptor tyrosine kinase 3
- Target synonymsErbB-3, FERLK, HER3, LCCS2, MDA-BF-1, VSCN1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3, receptor tyrosine-protein kinase erbB-3, human epidermal growth factor receptor 3, proto-oncogene-like protein c-ErbB-3, tyrosine kinase-type cell surface receptor HER3, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3
- HostMouse
- IsotypeIgG1
- Protein IDP21860
- Protein NameReceptor tyrosine-protein kinase erbB-3
- Scientific DescriptionErbB 3 (ERBB3) (Cytopl. Dom.) (incl. pos. control) mouse monoclonal antibody, clone 5A12, Purified
- ReactivityHuman
- UNSPSC12352203







![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with Her3 / ErbB3 antibody [C3], C-term (GTX100256) diluted at 1:500 and competitor's antibody (SC-285) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100256/GTX100256_43068_20190719_WB_competitor_watermark_w_23060100_544.webp)