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Western blot All lanes: ERGIC1 antibody at 2microg/ml + HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 33, 23, 18 kDa Observed band size: 33 kDa
Western blot All lanes: ERGIC1 antibody at 2microg/ml + HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 33, 23, 18 kDa Observed band size: 33 kDa
Western blot All lanes: ERGIC1 antibody at 2microg/ml + HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 33, 23, 18 kDa Observed band size: 33 kDa

ERGIC1 Antibody

CSB-PA842630LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetERGIC1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ERGIC1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57222
  • Target name
    ERGIC1
  • Target description
    endoplasmic reticulum-golgi intermediate compartment 1
  • Target synonyms
    AMC2, AMCN, ERGIC-32, ERGIC32, NET24, endoplasmic reticulum-Golgi intermediate compartment protein 1, ER-Golgi intermediate compartment 32 kDa protein, endoplasmic reticulum-golgi intermediate compartment (ERGIC) 1, endoplasmic reticulum-golgi intermediate compartment 32 kDa protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ969X5
  • Protein Name
    Endoplasmic reticulum-Golgi intermediate compartment protein 1
  • Scientific Description
    Possible role in transport between endoplasmic reticulum and Golgi.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Immunohistochemical staining of human cerebral cortex, liver, prostate and testis using Anti-ERGIC1 antibody HPA018666 (A) shows similar protein distribution across tissues to independent antibody HPA018900 (B).
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Figure 1. Western blot analysis of ERGIC1 using anti-ERGIC1 antibody (A11007-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERGIC1 antigen affinity purified polyclonal antibody (Catalog # A11007-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERGIC1 at approximately 35 kDa. The expected band size for ERGIC1 is at 33 kDa.
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