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Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ERK1/2(Thr202 + Tyr204) Polyclonal Antibody, Unconjugated (bs-3016R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ERK1/2(Thr202 + Tyr204) Polyclonal Antibody, Unconjugated (bs-3016R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ERK1/2(Thr202 + Tyr204) Polyclonal Antibody, Unconjugated (bs-3016R) at 1:200 overnight at 4°C, DAB staining.

phospho-ERK1/2 (Thr202 + Tyr204) Polyclonal Antibody

BS-3016R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMAPK3
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    ERK1/2(Thr202 + Tyr204) Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5595
  • Target name
    MAPK3
  • Target description
    mitogen-activated protein kinase 3
  • Target synonyms
    ERK1; ERK-1; ERT2; extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; insulin-stimulated MAP2 kinase; MAPK 1; microtubule-associated protein 2 kinase; mitogen-activated protein kinase 3; P44ERK1; p44-ERK1; P44MAPK; p44-MAPK; PRKM3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP27361
  • Protein Name
    Mitogen-activated protein kinase 3
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Down-regulation of Suv39h1 attenuates neointima formation after carotid artery injury in diabetic rats. Zhang J et al., 2020 Jan, J Cell Mol Med
    Read more
  • Clinical and biological significances of heat shock protein 90 (Hsp90) in human nasopharyngeal carcinoma cells and anti-cancer effects of Hsp90 inhibitor. Liu F et al., 2019 Dec, Biomed Pharmacother
    Read more
  • Vitamin B5 inhibit RANKL induced osteoclastogenesis and ovariectomy induced osteoporosis by scavenging ROS generation. Ma Q et al., 2019, Am J Transl Res
    Read more
  • LncRNA SNHG1 alleviates IL-1beta-induced osteoarthritis by inhibiting miR-16-5p-mediated p38 MAPK and NF-kappaB signaling pathways. Lei J et al., 2019 Sep 30, Biosci Rep
    Read more
  • Berberine suppresses mast cell-mediated allergic responses via regulating FcepsilonRI-mediated and MAPK signaling. Fu S et al., 2019 Jun, Int Immunopharmacol
    Read more
  • Ecdysterone Accelerates Healing of Radiation-Induced Oral Mucositis in Rats by Increasing Matrix Cell Proliferation. Li Y et al., 2019 Mar, Radiat Res
    Read more
  • Total flavones of fermentation broth by co-culture of Coprinus comatus and Morchella esculenta induces an anti-inflammatory effect on LPS-stimulated RAW264.7 macrophages cells via the MAPK signaling pathway. Zhao X et al., 2018 Dec, Microb Pathog
    Read more
  • Upregulation of long noncoding RNA CCAT1-L promotes epithelial-mesenchymal transition in gastric adenocarcinoma. Fang H et al., 2018, Onco Targets Ther
    Read more
  • Activation of the complement system in an osteosarcoma cell line promotes angiogenesis through enhanced production of growth factors. Jeon H et al., 2018 Apr 3, Sci Rep
    Read more
  • Seasonal Expression of Oxytocin and Oxytocin Receptor in the Scented Gland of Male Muskrat (Ondatra zibethicus). Zhang F et al., 2017 Nov 30, Sci Rep
    Read more

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