Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ERK1/2(Thr202 + Tyr204) Polyclonal Antibody, Unconjugated (bs-3016R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ERK1/2(Thr202 + Tyr204) Polyclonal Antibody, Unconjugated (bs-3016R) at 1:200 overnight at 4°C, DAB staining.
phospho-ERK1/2 (Thr202 + Tyr204) Polyclonal Antibody
BS-3016R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetMAPK3
Overview
- SupplierBioss
- Product Namephospho-ERK1/2 (Thr202 + Tyr204) Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID5595
- Target nameMAPK3
- Target descriptionmitogen-activated protein kinase 3
- Target synonymsERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, p44-ERK1, p44-MAPK, mitogen-activated protein kinase 3, MAPK 1, extracellular signal-regulated kinase 1, extracellular signal-related kinase 1, insulin-stimulated MAP2 kinase, microtubule-associated protein 2 kinase
- HostRabbit
- IsotypeIgG
- Protein IDP27361
- Protein NameMitogen-activated protein kinase 3
- Storage Instruction-20°C
- UNSPSC12352203
References
- Zhang J, Yang J, Xu C, et al. Down-regulation of Suv39h1 attenuates neointima formation after carotid artery injury in diabetic rats. J Cell Mol Med. 2020,24(1):973-983. doi: 10.1111/jcmm.14809Read this paper
- Liu F, Wang L, Yi S, et al. Clinical and biological significances of heat shock protein 90 (Hsp90) in human nasopharyngeal carcinoma cells and anti-cancer effects of Hsp90 inhibitor. Biomed Pharmacother. 2019,120:109533. doi: 10.1016/j.biopha.2019.109533Read this paper
- Ma Q, Liang M, Tang X, et al. Vitamin B5 inhibit RANKL induced osteoclastogenesis and ovariectomy induced osteoporosis by scavenging ROS generation. Am J Transl Res. 2019,11(8):5008-5018.Read this paper
- Lei J, Fu Y, Zhuang Y, et al. LncRNA SNHG1 alleviates IL-1β-induced osteoarthritis by inhibiting miR-16-5p-mediated p38 MAPK and NF-κB signaling pathways. Biosci Rep. 2019,39(9):pii: BSR20191523. doi: 10.1042/BSR20191523.Read this paper
- Fu S, Ni S, Wang D, et al. Berberine suppresses mast cell-mediated allergic responses via regulating FcɛRI-mediated and MAPK signaling. Int Immunopharmacol. 2019,71:1-6. doi: 10.1016/j.intimp.2019.02.041Read this paper
- Li Y, Ailing H, Jian P. Ecdysterone Accelerates Healing of Radiation-Induced Oral Mucositis in Rats by Increasing Matrix Cell Proliferation. Radiat Res. 2019,191(3):237-244. doi: 10.1667/RR15171.1Read this paper
- Zhao X, Zou X, Li Q, et al. Total flavones of fermentation broth by co-culture of Coprinus comatus and Morchella esculenta induces an anti-inflammatory effect on LPS-stimulated RAW264.7 macrophages cells via the MAPK signaling pathway. Microb Pathog. 2018,125:431-437. doi: 10.1016/j.micpath.2018.10.008Read this paper
- Fang H, Liu HM, Wu WH, et al. Upregulation of long noncoding RNA CCAT1-L promotes epithelial-mesenchymal transition in gastric adenocarcinoma. Onco Targets Ther. 2018,11:5647-5655. doi: 10.2147/OTT.S170553Read this paper
- Jeon H, Han SR, Lee S, et al. Activation of the complement system in an osteosarcoma cell line promotes angiogenesis through enhanced production of growth factors. Sci Rep. 2018,8(1):5415. doi: 10.1038/s41598-018-23851-zRead this paper
- Zhang F, Liu Q, Wang Z, et al. Seasonal Expression of Oxytocin and Oxytocin Receptor in the Scented Gland of Male Muskrat (Ondatra zibethicus). Sci Rep. 2017,7(1):16627. doi: 10.1038/s41598-017-16973-3Read this paper
Datasheet
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