More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/ESM1 Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ESM1 Polyclonal Antibody, Unconjugated (bs-3615R) at 1:400 overnight at 4°C, followed by a FITC conjugated secondary antibody for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ESM1 Polyclonal Antibody, Unconjugated (bs-3615R) at 1:400 overnight at 4°C, followed by a FITC conjugated secondary antibody for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ESM1 Polyclonal Antibody, Unconjugated (bs-3615R) at 1:400 overnight at 4°C, followed by a FITC conjugated secondary antibody for 90 minutes, and DAPI for nuclei staining.

ESM1 Polyclonal Antibody

BS-3615R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetESM1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss
  • Product Name
    ESM1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID11082
  • Target name
    ESM1
  • Target description
    endothelial cell specific molecule 1
  • Target synonyms
    endocan, endothelial cell-specific molecule 1, ESM-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NQ30
  • Protein Name
    Endothelial cell-specific molecule 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Zhao H, Guo Y, Sun Y, et al. miR-181a/b-5p ameliorates inflammatory response in monocrotaline-induced pulmonary arterial hypertension by targeting endocan. J Cell Physiol. 2020,235(5):4422-4433. doi: 10.1002/jcp.29318
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA007825HA01HU at dilution of 1:100
Product group Antibodies
Cusabio
ESM1 AntibodyCSB-PA007825HA01HU
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
TargetESM1
  • SizePrice
Figure 1. Western blot analysis of ESM1 using anti-ESM1 antibody (A04169-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human ESM1 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESM1 antigen affinity purified polyclonal antibody (Catalog # A04169-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ESM1 at approximately 25KD. The expected band size for ESM1 is at 20KD.
Product group Antibodies
Boster Bio
Anti-ESM1 Antibody Picoband(r)A04169-1-CARRIER-FREE
ApplicationsWestern Blot, ELISA
TargetESM1
  • SizePrice
Product group Antibodies
Biomatik
Esm1 Polyclonal AntibodyCAC10362
ApplicationsELISA, ImmunoHistoChemistry
TargetESM1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ESM1 / Endocan AntibodyLS-C830287
ApplicationsELISA, ImmunoHistoChemistry
TargetESM1
  • SizePrice