Anti-Esrp-1/2 antibody by western blot shows detection in 293T cell extracts. Lane 1: GFP-transfected. Lane 2: Esrp-1 transfected (arrow). Lane 3: Esrp-2 transfected. Each lane contains approximately 5 μg of lysate. Primary antibody was used at a 1:1000 dilution in PBS-T plus milk, and reacted for 1hr at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse secondary antibody for 1hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers.
![WB analysis of transfected 293T whole cell lysate using GTX48760 Esrp-1/2 antibody [23A7.C9]. Lane 1 : GFP Lane 2 : Espr-1 Lane 3 : Esrp-2 Loading : 5 μg Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX48760/GTX48760_20240423_WB_298_24042320_859.webp "WB analysis of transfected 293T whole cell lysate using GTX48760 Esrp-1/2 antibody [23A7.C9]. Lane 1 : GFP Lane 2 : Espr-1 Lane 3 : Esrp-2 Loading : 5 μg Dilution : 1:1000")
Anti-Esrp-1/2 antibody by western blot shows detection in 293T cell extracts. Lane 1: GFP-transfected. Lane 2: Esrp-1 transfected (arrow). Lane 3: Esrp-2 transfected. Each lane contains approximately 5 μg of lysate. Primary antibody was used at a 1:1000 dilution in PBS-T plus milk, and reacted for 1hr at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse secondary antibody for 1hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers.
Esrp-1/2 antibody [23A7.C9]
GTX48760
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityMouse
Overview
- SupplierGeneTex
- Product NameEsrp-1/2 antibody [23A7.C9]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000. ELISA: 1:750000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID23A7.C9
- Concentration1 mg/ml
- ConjugateUnconjugated
- HostMouse
- IsotypeIgG1
- Scientific DescriptionEpithelial splicing regulatory protein-1 (Esrp-1) and Esrp-2 are mRNA splicing factors that regulate the formation of epithelial cell-specific isoforms. They specifically regulate the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-1 and -2 act by directly binding specific sequences in mRNAs. They bind the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.
- ReactivityMouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161