Anti-ESRP-1 by western blot shows detection of ESRP-1 in transfected 293T cell extracts (lane 2, arrowhead). Lanes 1 and 3 contain GFP-transfected- and ESRP2-transfected 293T cell lysates, respectively. Briefly, each lane contains approximately 5 μg ysate. Primary antibody was used at a 1:1000 dilution in PBST plus milk, and reacted for 1hr at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse secondary antibody for 1hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers.
![WB analysis of transfected 293T whole cell lysate using GTX48761 ESRP1 antibody [27H12]. Lane 1 : GFP Lane 2 : ESRP1 Lane 3 : ESRP2 Loading : 5 μg Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX48761/GTX48761_20240423_WB_299_24042320_294.webp "WB analysis of transfected 293T whole cell lysate using GTX48761 ESRP1 antibody [27H12]. Lane 1 : GFP Lane 2 : ESRP1 Lane 3 : ESRP2 Loading : 5 μg Dilution : 1:1000")
Anti-ESRP-1 by western blot shows detection of ESRP-1 in transfected 293T cell extracts (lane 2, arrowhead). Lanes 1 and 3 contain GFP-transfected- and ESRP2-transfected 293T cell lysates, respectively. Briefly, each lane contains approximately 5 μg ysate. Primary antibody was used at a 1:1000 dilution in PBST plus milk, and reacted for 1hr at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse secondary antibody for 1hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers.
ESRP1 antibody [27H12]
GTX48761
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityMouse
TargetEsrp1
Overview
- SupplierGeneTex
- Product NameESRP1 antibody [27H12]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000. ELISA: 1:750000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID27H12
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID207920
- Target nameEsrp1
- Target descriptionepithelial splicing regulatory protein 1
- Target synonyms2210008M09Rik, A630065D16, Rbm35a, epithelial splicing regulatory protein 1, RNA binding motif protein 35A
- HostMouse
- IsotypeIgG2b
- Protein IDQ3US41
- Protein NameEpithelial splicing regulatory protein 1
- Scientific DescriptionEpithelial splicing regulatory protein 1 (Esrp-1) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). EsrpP-1 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.
- ReactivityMouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161