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ESRRG Antibody

CSB-PA007837LA01HU
Cusabio
ApplicationsELISA
Product group Antibodies
TargetESRRG
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Overview

  • Supplier
    Cusabio
  • Product Name
    ESRRG Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2104
  • Target name
    ESRRG
  • Target description
    estrogen related receptor gamma
  • Target synonyms
    ERR gamma-2; ERR3; ERRg; ERRgamma; ERR-gamma; estrogen receptor-related protein 3; estrogen-related receptor gamma; NR3B3; nuclear receptor subfamily 3 group B member 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP62508
  • Protein Name
    Estrogen-related receptor gamma
  • Scientific Description
    Orphan receptor that acts as transcription activator in the absence of bound ligand. Binds specifically to an estrogen response element and activates reporter genes controlled by estrogen response elements. Induces the expression of PERM1 in the skeletal muscle.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ESRRG using anti-ESRRG antibody (A01470-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PANC-1 whole cell lysates, Lane 2: human 22RV1 whole cell lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human SW620 whole cell lysates, Lane 5: mouse SP20 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESRRG antigen affinity purified polyclonal antibody (Catalog # A01470-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ESRRG at approximately 51KD. The expected band size for ESRRG is at 51KD.
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