
Tissue/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Estrogen Receptor alpha) polyclonal Antibody, Unconjugated (bs-0122R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Estrogen Receptor alpha Antibody
BS-0122R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
TargetEsr1
Overview
- SupplierBioss
- Product NameEstrogen Receptor alpha Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID24890
- Target nameEsr1
- Target descriptionestrogen receptor 1
- Target synonymsER-alpha, Esr, RNESTROR, estrogen receptor, C-terminally-truncated estrogen receptor alpha product-1, Er alpha, estradiol receptor, estrogen receptor 1 (alpha), estrogen receptor alpha i45aL, estrogen receptor alpha i45aS, estrogen receptor alpha i45c, estrogen receptor alpha i56a, estrogen receptor alpha i56b, estrogen receptor alpha i56c, estrogen receptor alpha i56dL, estrogen receptor alpha i56dS, estrogen receptor alpha i56e, estrogen receptor alpha variant delta 4, estrogen receptor protein, nuclear receptor subfamily 3 group A member 1, trunctaed estrogen receptor product-1
- HostRabbit
- IsotypeIgG
- Protein IDP06211
- Protein NameEstrogen receptor
- Storage Instruction-20°C
- UNSPSC12352203

