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Tissue/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Estrogen Receptor alpha) polyclonal Antibody, Unconjugated (bs-0122R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Estrogen Receptor alpha) polyclonal Antibody, Unconjugated (bs-0122R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Estrogen Receptor alpha) polyclonal Antibody, Unconjugated (bs-0122R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Estrogen Receptor alpha Antibody

BS-0122R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
TargetEsr1
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Overview

  • Supplier
    Bioss
  • Product Name
    Estrogen Receptor alpha Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID24890
  • Target name
    Esr1
  • Target description
    estrogen receptor 1
  • Target synonyms
    ER-alpha, Esr, RNESTROR, estrogen receptor, C-terminally-truncated estrogen receptor alpha product-1, Er alpha, estradiol receptor, estrogen receptor 1 (alpha), estrogen receptor alpha i45aL, estrogen receptor alpha i45aS, estrogen receptor alpha i45c, estrogen receptor alpha i56a, estrogen receptor alpha i56b, estrogen receptor alpha i56c, estrogen receptor alpha i56dL, estrogen receptor alpha i56dS, estrogen receptor alpha i56e, estrogen receptor alpha variant delta 4, estrogen receptor protein, nuclear receptor subfamily 3 group A member 1, trunctaed estrogen receptor product-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06211
  • Protein Name
    Estrogen receptor
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203