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Lane 1: MOLT-4 Cell lysates; probed with ETS1 (1C1) Monoclonal Antibody, unconjugated (bsm-51344M) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: MOLT-4 Cell lysates; probed with ETS1 (1C1) Monoclonal Antibody, unconjugated (bsm-51344M) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: MOLT-4 Cell lysates; probed with ETS1 (1C1) Monoclonal Antibody, unconjugated (bsm-51344M) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

ETS1 (1C1) Monoclonal Antibody

BSM-51344M
Bioss
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityHuman
TargetETS1
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Overview

  • Supplier
    Bioss
  • Product Name
    ETS1 (1C1) Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1C1
  • Concentration
    0.5 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID2113
  • Target name
    ETS1
  • Target description
    ETS proto-oncogene 1, transcription factor
  • Target synonyms
    ETS-1, EWSR2, c-ets-1, p54, protein C-ets-1, Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1, v-ets avian erythroblastosis virus E2 oncogene homolog 1, v-ets avian erythroblastosis virus E26 oncogene homolog 1
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP14921
  • Protein Name
    Protein C-ets-1
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ETS1 using anti-ETS1 antibody (A00931-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: NIH3T3 whole Cell lysates, Lane 2: A375 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ETS1 antigen affinity purified polyclonal antibody (Catalog # A00931-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ETS1 at approximately 54KD. The expected band size for ETS1 is at 54KD.
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