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Cross-reactivity assessment of ETS1 Mouse Monoclonal Antibody AE00173 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
Cross-reactivity assessment of ETS1 Mouse Monoclonal Antibody AE00173 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
Cross-reactivity assessment of ETS1 Mouse Monoclonal Antibody AE00173 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.

ETS1 Monoclonal Antibody AE00173

AE00173
Aeonian Biotech
TargetETS1
Product group Antibodies

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Overview

  • Supplier
    Aeonian Biotech
  • Product Name
    ETS1 Monoclonal Antibody AE00173
  • Delivery Days Customer
    9
  • Applications Supplier
    IHC, PA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ETS1/1282
  • Conjugate
    Unconjugated
  • Gene ID2113
  • Target name
    ETS1
  • Target description
    ETS proto-oncogene 1, transcription factor
  • Target synonyms
    ETS-1, EWSR2, c-ets-1, p54, protein C-ets-1, Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1, v-ets avian erythroblastosis virus E2 oncogene homolog 1, v-ets avian erythroblastosis virus E26 oncogene homolog 1
  • Host
    Mouse
  • Isotype
    IgG2b kappa
  • Protein IDP14921
  • Protein Name
    Protein C-ets-1
  • Scientific Description
    ETS1 Monoclonal Antibody AE00173
  • Shelf life instruction
    Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
  • Reactivity Supplier
    Human
  • Storage Instruction
    2-8°C
  • UNSPSC
    12352203

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MSDS

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Figure 1. Western blot analysis of ETS1 using anti-ETS1 antibody (A00931-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: NIH3T3 whole Cell lysates, Lane 2: A375 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ETS1 antigen affinity purified polyclonal antibody (Catalog # A00931-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ETS1 at approximately 54KD. The expected band size for ETS1 is at 54KD.
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Mouse spleen lysates probed with Rabbit Anti-ETS1 Polyclonal Antibody, Unconjugated (bs-1285R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
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Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.
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Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ETS1 antibody [N3C1], Internal (GTX100639) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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