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ProductsBack/ETS1 Mouse Monoclonal Antibody [Clone ID: 10D2]
Western blot analysis using ETS1 antibody Cat.-No AM06416SU-N against Jurkat (Lane 1), HepG2 (Lane 2) and ETS1-hIgGFc transfected HEK293 (Lane 3) cell lysate.
Western blot analysis using ETS1 antibody Cat.-No AM06416SU-N against Jurkat (Lane 1), HepG2 (Lane 2) and ETS1-hIgGFc transfected HEK293 (Lane 3) cell lysate.
Western blot analysis using ETS1 antibody Cat.-No AM06416SU-N against Jurkat (Lane 1), HepG2 (Lane 2) and ETS1-hIgGFc transfected HEK293 (Lane 3) cell lysate.

ETS1 Mouse Monoclonal Antibody [Clone ID: 10D2]

AM06416SU-N
OriGene
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetETS1
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    ETS1 Mouse Monoclonal Antibody [Clone ID: 10D2]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    10D2
  • Gene ID2113
  • Target name
    ETS1
  • Target description
    ETS proto-oncogene 1, transcription factor
  • Target synonyms
    ETS-1, EWSR2, c-ets-1, p54, protein C-ets-1, Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1, v-ets avian erythroblastosis virus E2 oncogene homolog 1, v-ets avian erythroblastosis virus E26 oncogene homolog 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP14921
  • Protein Name
    Protein C-ets-1
  • Scientific Description
    ETS1 mouse monoclonal antibody, clone 10D2, Ascites
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of ETS1 using anti-ETS1 antibody (A00931-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: NIH3T3 whole Cell lysates, Lane 2: A375 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ETS1 antigen affinity purified polyclonal antibody (Catalog # A00931-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ETS1 at approximately 54KD. The expected band size for ETS1 is at 54KD.
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