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ProductsBack/Eukaryotic Mouse Interleukin 27 (IL27)

Eukaryotic Mouse Interleukin 27 (IL27)

RPU59266
Biomatik
ApplicationsWestern Blot, Other Application
Product group Proteins / Signaling Molecules
Protein IDO35228
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Overview

  • Supplier
    Biomatik
  • Product Name
    Eukaryotic Interleukin 27 (IL27)
  • Delivery Days Customer
    12
  • Applications
    Western Blot, Other Application
  • Applications Supplier
    Positive Control; Immunogen; SDS-PAGE; WB.
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Estimated Purity
    >90%
  • Protein IDO35228
  • Protein Name
    Interleukin-27 subunit beta
  • Scientific Description
    Eukaryotic Mouse Interleukin 27 (IL27) is a purified Eukaryotic Protein.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352202

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IL-27 (mouse):Fc-KIH (human) (rec.) (#AG-40B-0236) (Heterodimer) is more active than an IL-27 (mouse):Fc from a competitor (Dimer of Heterodimer). Method: 5x104 HepG2 cells (a human hepatocellular carcinoma cell line) are treated with IL-27 (mouse):Fc-KIH (human) (rec.) (#AG-40B-0236, AdipoGen) or from IL-27 (mouse):Fc (human) from a competitor at different concentrations indicated on the figure for 15 minutes. Cells are lysed and cell extracts are separated by SDS-PAGE (12.5%) under reducing conditions, transferred to nitrocellulose overnight and incubated with anti-Phospho-Stat3 (Tyr705) (human), Rabbit Monoclonal (RM261) (#REV-31-1142-00) (1/1000) or anti-Stat3, Rabbit Monoclonal (RM465) (#REV-31-1357-00) (1/1000). After the addition of an anti-rabbit secondary antibody coupled to HRP (1/10000), proteins are visualized by a chemiluminescence detection system. A) Western Blot, B) Quantification figure of the Western Blot.
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