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ProductsBack/EVL antibody, Internal
WB analysis of Jurkat lysate using GTX47560 EVL antibody, Internal. Dilution : 1microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of Jurkat lysate using GTX47560 EVL antibody, Internal. Dilution : 1microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of Jurkat lysate using GTX47560 EVL antibody, Internal. Dilution : 1microg/ml Loading : 35microg protein in RIPA buffer

EVL antibody, Internal

GTX47560
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetEVL
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Overview

  • Supplier
    GeneTex
  • Product Name
    EVL antibody, Internal
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1-3microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID51466
  • Target name
    EVL
  • Target description
    Enah/Vasp-like
  • Target synonyms
    ena/vasodilator-stimulated phosphoprotein-like; ena/VASP-like protein; epididymis secretory sperm binding protein; RNB6
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ9UI08
  • Protein Name
    Ena/VASP-like protein
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EVL using anti-EVL antibody (A02568-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CCRF-CEM whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EVL antigen affinity purified polyclonal antibody (Catalog # A02568-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EVL at approximately 50 kDa. The expected band size for EVL is at 50 kDa.
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