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ProductsBack/EXOSC2 / RRP4 Antibody

EXOSC2 / RRP4 Antibody

LS-C497159
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetEXOSC2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    EXOSC2 / RRP4 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 29kDa/30kDa/32kDa, while the observed MW by Western blot was 35kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 29kDa/30kDa/32kDa, while the observed MW by Western blot was 35kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23404
  • Target name
    EXOSC2
  • Target description
    exosome component 2
  • Target synonyms
    RRP4, Rrp4p, SHRF, hRrp4p, p7, exosome complex component RRP4, exosome complex exonuclease RRP4, homolog of yeast RRP4 (ribosomal RNA processing 4), 3' 5' exoribonuclease (RRP4), homolog of yeast RRP4 (ribosomal RNA processing 4), 3'-5'-exoribonuclease, ribosomal RNA-processing protein 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RRP4/EXOSC2 using anti-RRP4/EXOSC2 antibody (A10119-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRP4/EXOSC2 antigen affinity purified polyclonal antibody (Catalog # A10119-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRP4/EXOSC2 at approximately 33 kDa. The expected band size for RRP4/EXOSC2 is at 33 kDa.
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