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ProductsBack/EXOSC8 (H-8): m-IgGkappa BP-HRP Bundle

EXOSC8 (H-8): m-IgGkappa BP-HRP Bundle

SC-523779
Santa Cruz Biotechnology
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetEXOSC8
1 kit
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    EXOSC8 (H-8): m-IgGkappa BP-HRP Bundle
  • Delivery Days Customer
    5
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID11340
  • Target name
    EXOSC8
  • Target description
    exosome component 8
  • Target synonyms
    CIP3, EAP2, OIP2, PCH1C, RRP43, Rrp43p, bA421P11.3, p9, exosome complex component RRP43, CBP-interacting protein 3, OIP-2, Opa interacting protein 2, exosome complex exonuclease RRP43, ribosomal RNA-processing protein 43
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of EXOSC8 using anti-EXOSC8 antibody (A09191-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EXOSC8 antigen affinity purified polyclonal antibody (Catalog # A09191-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EXOSC8 at approximately 35 kDa. The expected band size for EXOSC8 is at 35 kDa.
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