F-Testo(Free Testosterone) ELISA Kit

Biorbyt

F-Testo(Free Testosterone) ELISA Kit

10

standard: 2000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with F-TESTO protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to F-TESTO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of F-TESTO in the samples is then determined by comparing the OD of the samples to the standard curve

31.25-2000 pg/mL

18.76 pg/mL

Research Use Only

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with FreeTestosterone(F-TESTO) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to FreeTestosterone(F-TESTO). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FreeTestosterone(F-TESTO) in the samples is then determined by comparing the OD of the samples to the standard curve.

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