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ProductsBack/F4/80 antibody [CI:A3-1] (Low endotoxin, azide free)
IHC-Fr analysis of mouse spleen tissue using GTX41205 F4/80 antibody at 1/100
IHC-Fr analysis of mouse spleen tissue using GTX41205 F4/80 antibody at 1/100
IHC-Fr analysis of mouse spleen tissue using GTX41205 F4/80 antibody at 1/100

F4/80 antibody [CI:A3-1] (Low endotoxin, azide free)

GTX41205
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking, RadioImmunoAssay
Product group Antibodies
ReactivityHuman, Mouse
TargetAdgre1
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Overview

  • Supplier
    GeneTex
  • Product Name
    F4/80 antibody [CI:A3-1] (Low endotoxin, azide free)
  • Delivery Days Customer
    9
  • Application Supplier Note
    FCM: 1/100-1/200. Neutralizing/Inhibition: . *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking, RadioImmunoAssay
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CI:A3-1
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID13733
  • Target name
    Adgre1
  • Target description
    adhesion G protein-coupled receptor E1
  • Target synonyms
    DD7A5-7, EGF-TM7, Emr1, F4/80, Gpf480, Ly71, TM7LN3, adhesion G protein-coupled receptor E1, EGF-like module containing, mucin-like, hormone receptor-like sequence 1, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 hormone receptor, cell surface glycoprotein F4/80
  • Host
    Rat
  • Isotype
    IgG2b
  • Protein IDQ61549
  • Protein Name
    Adhesion G protein-coupled receptor E1
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

References

  • Targeting IL-17B-IL-17RB signaling with an anti-IL-17RB antibody blocks pancreatic cancer metastasis by silencing multiple chemokines. Wu HH et al., 2015 Mar 9, J Exp Med
    Read this paper
  • CCN1 expression in hepatocytes contributes to macrophage infiltration in nonalcoholic fatty liver disease in mice. Bian Z et al., 2013 Jan, J Lipid Res
    Read this paper
  • Benzo[a]pyrene potentiates the pathogenesis of abdominal aortic aneurysms in apolipoprotein E knockout mice. Prins PA et al., 2012, Cell Physiol Biochem
    Read this paper
  • Zinc oxide nanoparticles-induced intercellular adhesion molecule 1 expression requires Rac1/Cdc42, mixed lineage kinase 3, and c-Jun N-terminal kinase activation in endothelial cells. Li CH et al., 2012 Mar, Toxicol Sci
    Read this paper
  • Abcg2 labels multiple cell types in skeletal muscle and participates in muscle regeneration. Doyle MJ et al., 2011 Oct 3, J Cell Biol
    Read this paper
  • Quantitative estimates of the variability of in vivo sonographic measurements of the mouse aorta for studies of abdominal aortic aneurysms and related arterial diseases. Sampson UK et al., 2011 Jun, J Ultrasound Med
    Read this paper
  • Contribution of macrophages to angiogenesis induced by vascular endothelial growth factor receptor-3-specific ligands. Chung ES et al., 2009 Nov, Am J Pathol
    Read this paper

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Figure 1. IHC analysis of F4/80/Adgre1 using anti-F4/80/Adgre1 antibody (A08751). F4/80/Adgre1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 microg/ml rabbit anti-F4/80/Adgre1 Antibody (A08751) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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FACS analysis of mouse C57Bl/6 bone marrow cells using GTX01470-10 F4/80 antibody [BM8.1] (PE-Cy7). Right panel : co-stained with F4/80 antibody [BM8.1] (PE-Cy7) and Mouse CD11b antibody (APC) Left panel : co-stained with isotype control and Mouse CD11b antibody (APC) antibody amount : 0.25 μg (5 μl)
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FACS analysis of mouse C57Bl/6 bone marrow cells using GTX01470-11 F4/80 antibody [BM8.1] (PerCP-Cy5.5). Right panel : co-stained with F4/80 antibody [BM8.1] (PerCP-Cy5.5) and Mouse CD11b antibody (APC) Left panel : co-stained with isotype control and Mouse CD11b antibody (APC) antibody amount : 0.125 μg (5 μl)
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