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F8 Polyclonal Antibody

CAC08419
Biomatik
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetF8
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Overview

  • Supplier
    Biomatik
  • Product Name
    F8 Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC, IF; Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2157
  • Target name
    F8
  • Target description
    coagulation factor VIII
  • Target synonyms
    AHF, DXS1253E, F8B, F8C, FVIII, HEMA, THPH13, coagulation factor VIII, antihemophilic factor, coagulation factor VIII A1 domain, coagulation factor VIII C2 domain, coagulation factor VIII, procoagulant component, coagulation factor VIIIc, factor VIII F8B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00451
  • Protein Name
    Coagulation factor VIII
  • Scientific Description
    The F8 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, IHC, IF.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Factor VIII using anti-Factor VIII antibody (PA2061). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: human Hela whole cell lysates, Lane 7: human HepG2 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor VIII antigen affinity purified polyclonal antibody (Catalog # PA2061) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Factor VIII at approximately 92KD. The expected band size for Factor VIII is at 92KD.
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