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ProductsBack/FADS1 Rabbit Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using FADS1 antibody (TA376078) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Enhanced Kit . - Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using FADS1 antibody (TA376078) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Enhanced Kit . - Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using FADS1 antibody (TA376078) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Enhanced Kit . - Exposure time: 90s.

FADS1 Rabbit Polyclonal Antibody

TA376078
OriGene
ApplicationsWestern Blot
Product group Antibodies
TargetFADS1
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    FADS1 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID3992
  • Target name
    FADS1
  • Target description
    fatty acid desaturase 1
  • Target synonyms
    D5D, FADS6, FADSD5, LLCDL1, TU12, acyl-CoA (8-3)-desaturase, delta(5) desaturase, delta-5 fatty acid desaturase, linoleoyl-CoA desaturase (delta-6-desaturase)-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60427
  • Protein Name
    Fatty acid desaturase 1
  • Scientific Description
    FADS1 Rabbit polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of FADS1 using anti-FADS1 antibody (A02535-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FADS1 antigen affinity purified polyclonal antibody (Catalog # A02535-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FADS1 at approximately 52 kDa. The expected band size for FADS1 is at 52 kDa.
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