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FADS1 Recombinant Antibody, HRP Conjugated

BSM-62440R-HRP
Bioss Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetFADS1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    FADS1 Recombinant Antibody, HRP Conjugated
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    HRP
  • Gene ID3992
  • Target name
    FADS1
  • Target description
    fatty acid desaturase 1
  • Target synonyms
    acyl-CoA (8-3)-desaturase; D5D; delta(5) desaturase; delta-5 fatty acid desaturase; FADS6; FADSD5; linoleoyl-CoA desaturase (delta-6-desaturase)-like 1; LLCDL1; TU12
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60427
  • Protein Name
    Fatty acid desaturase 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FADS1 using anti-FADS1 antibody (A02535-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FADS1 antigen affinity purified polyclonal antibody (Catalog # A02535-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FADS1 at approximately 52 kDa. The expected band size for FADS1 is at 52 kDa.
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