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ProductsBack/FAM129B antibody

FAM129B antibody

GTX78915
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetNIBAN2
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Overview

  • Supplier
    GeneTex
  • Product Name
    FAM129B antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64855
  • Target name
    NIBAN2
  • Target description
    niban apoptosis regulator 2
  • Target synonyms
    C9orf88, FAM129B, MEG-3, MINERVA, OC58, bA356B19.6, protein Niban 2, family with sequence similarity 129 member B, melanoma invasion by ERK, niban-like protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96TA1
  • Protein Name
    Protein Niban 2
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of FAM129B/NIBAN2 using anti-FAM129B/NIBAN2 antibody (A09391-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: rat stomach tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse stomach tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAM129B/NIBAN2 antigen affinity purified polyclonal antibody (Catalog # A09391-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAM129B/NIBAN2 at approximately 95 kDa. The expected band size for FAM129B/NIBAN2 is at 95 kDa.
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