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ProductsBack/FAM129B antibody
WB analysis of NIH3T3, 3Y1E, and DU145 cell lysates using GTX78915 FAM129B antibody.
WB analysis of NIH3T3, 3Y1E, and DU145 cell lysates using GTX78915 FAM129B antibody.
WB analysis of NIH3T3, 3Y1E, and DU145 cell lysates using GTX78915 FAM129B antibody.

FAM129B antibody

GTX78915
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNIBAN2
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Overview

  • Supplier
    GeneTex
  • Product Name
    FAM129B antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64855
  • Target name
    NIBAN2
  • Target description
    niban apoptosis regulator 2
  • Target synonyms
    C9orf88, FAM129B, MEG-3, MINERVA, OC58, bA356B19.6, protein Niban 2, family with sequence similarity 129 member B, melanoma invasion by ERK, niban-like protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96TA1
  • Protein Name
    Protein Niban 2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FAM129B/NIBAN2 using anti-FAM129B/NIBAN2 antibody (A09391-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: rat stomach tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse stomach tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAM129B/NIBAN2 antigen affinity purified polyclonal antibody (Catalog # A09391-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAM129B/NIBAN2 at approximately 95 kDa. The expected band size for FAM129B/NIBAN2 is at 95 kDa.
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