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FAM40B Antibody

LS-C665508
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSTRIP2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    FAM40B Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID57464
  • Target name
    STRIP2
  • Target description
    striatin interacting protein 2
  • Target synonyms
    FAM40B, FAR11B, striatin-interacting protein 2, FAR11 factor arrest 11 homolog B, family with sequence similarity 40, member B, homolog of yeast FAR11 protein 2, protein FAM40B
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FAM40B/STRIP2 using anti-FAM40B/STRIP2 antibody (A12139). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAM40B/STRIP2 antigen affinity purified polyclonal antibody (Catalog # A12139) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAM40B/STRIP2 at approximately 100 kDa. The expected band size for FAM40B/STRIP2 is at 95 kDa.
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