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WB analysis of various sample lysates using GTX66562 FAM65B antibody. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66562 FAM65B antibody. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX66562 FAM65B antibody. Dilution : 1:3000 Loading : 25microg per lane

FAM65B antibody

GTX66562
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetRIPOR2
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Overview

  • Supplier
    GeneTex
  • Product Name
    FAM65B antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9750
  • Target name
    RIPOR2
  • Target description
    RHO family interacting cell polarization regulator 2
  • Target synonyms
    C6orf32, DFNA21, DFNB104, DIFF40, DIFF48, FAM65B, MYONAP, PL48, rho family-interacting cell polarization regulator 2, family with sequence similarity 65 member B, myogenesis-related and NCAM-associated protein homolog, protein FAM65B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y4F9
  • Protein Name
    Rho family-interacting cell polarization regulator 2
  • Scientific Description
    This gene encodes an atypical inhibitor of the small G protein RhoA. Inhibition of RhoA activity by the encoded protein mediates myoblast fusion and polarization of T cells and neutrophils. The encoded protein is a component of hair cell stereocilia that is essential for hearing. A splice site mutation in this gene results in hearing loss in human patients. [provided by RefSeq, Sep 2016]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Lane 1: Bone lysates probed with FAM65B Polyclonal Antibody, Unconjugated (bs-12370R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of FAM65B/RIPOR2 using anti-FAM65B/RIPOR2 antibody (A32101-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAM65B/RIPOR2 antigen affinity purified polyclonal antibody (Catalog # A32101-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAM65B/RIPOR2 at approximately 120 kDa. The expected band size for FAM65B/RIPOR2 is at 119 kDa.
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