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ProductsBack/FAM70B (E-4)

FAM70B (E-4)

SC-514100
Santa Cruz Biotechnology
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTmem255b
200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    FAM70B (E-4)
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID272465
  • Target name
    Tmem255b
  • Target description
    transmembrane protein 255B
  • Target synonyms
    Fam70b, Gm687, transmembrane protein 255B, family with sequence similarity 70, member B, protein FAM70B
  • Host
    Mouse
  • Isotype
    IgM
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TMEM255B using anti-TMEM255B antibody (A18562). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse heart tissue lysates, Lane 2: mouse testis tissue lysates, Lane 3: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM255B antigen affinity purified polyclonal antibody (Catalog # A18562)at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM255B at approximately 45KD. The expected band size for TMEM255B is at 45KD.
Product group Antibodies
Boster Bio
Anti-Tmem255b Antibody Picoband(r)A18562-CARRIER-FREE
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetTmem255b
  • SizePrice