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ICC/IF analysis of NIH-3T3 cells using GTX53977 FANCC antibody. Red : Primary antibody Blue : DAPI Dilution : 1:100
ICC/IF analysis of NIH-3T3 cells using GTX53977 FANCC antibody. Red : Primary antibody Blue : DAPI Dilution : 1:100
ICC/IF analysis of NIH-3T3 cells using GTX53977 FANCC antibody. Red : Primary antibody Blue : DAPI Dilution : 1:100

FANCC antibody

GTX53977
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetFANCC
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Overview

  • Supplier
    GeneTex
  • Product Name
    FANCC antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2176
  • Target name
    FANCC
  • Target description
    FA complementation group C
  • Target synonyms
    FA3; FAC; FACC; Fanconi anemia complementation group C; Fanconi anemia group C protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ00597
  • Protein Name
    Fanconi anemia group C protein
  • Scientific Description
    The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group C. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Jurkat whole cell lysate, NIH/3T3 whole cell lysate All lanes: FANCC antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 64 kDa Observed band size: 64 kDa
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Figure 1. Western blot analysis of FANCC using anti-FANCC antibody (A02387-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FANCC antigen affinity purified polyclonal antibody (Catalog # A02387-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FANCC at approximately 63 kDa. The expected band size for FANCC is at 63 kDa.
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