Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with FANCJ antibody [N1N2], N-term (GTX100072) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
![FANCJ antibody [N1N2], N-term detects BRIP1 protein at nucleus on SAS xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SAS xenograft. FANCJ antibody [N1N2], N-term (GTX100072) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX100072/GTX100072_39384_IHC_w_23053123_266.webp "FANCJ antibody [N1N2], N-term detects BRIP1 protein at nucleus on SAS xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SAS xenograft. FANCJ antibody [N1N2], N-term (GTX100072) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
![FANCJ antibody [N1N2], N-term detects BRIP1 protein at cytoplasm by immunofluorescent analysis. Sample: 293T cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: BRIP1 protein stained by FANCJ antibody [N1N2], N-term (GTX100072) diluted at 1:500. Blue: Hoechst 33343 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100072/GTX100072_39384_IFA_w_23053123_760.webp "FANCJ antibody [N1N2], N-term detects BRIP1 protein at cytoplasm by immunofluorescent analysis. Sample: 293T cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: BRIP1 protein stained by FANCJ antibody [N1N2], N-term (GTX100072) diluted at 1:500. Blue: Hoechst 33343 staining.")
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FANCJ antibody [N1N2], N-term (GTX100072) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100072/GTX100072_39384_20230609_WB_23061400_113.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FANCJ antibody [N1N2], N-term (GTX100072) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with FANCJ antibody [N1N2], N-term (GTX100072) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
FANCJ antibody [N1N2], N-term
GTX100072
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetBRIP1
Overview
- SupplierGeneTex
- Product NameFANCJ antibody [N1N2], N-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID83990
- Target nameBRIP1
- Target descriptionBRCA1 interacting DNA helicase 1
- Target synonymsBACH1, FANCJ, OF, Fanconi anemia group J protein, ATP-dependent RNA helicase BRIP1, BRCA1 interacting helicase 1, BRCA1 interacting protein C-terminal helicase 1, BRCA1-associated C-terminal helicase 1, BRCA1-binding helicase-like protein BACH1, BRCA1/BRCA2-associated helicase 1, DNA 5'-3' helicase FANCJ, FANCJ helicase
- HostRabbit
- IsotypeIgG
- Protein IDQ9BX63
- Protein NameFanconi anemia group J protein
- Scientific DescriptionThe protein encoded by this gene is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). This gene may be a target of germline cancer-inducing mutations. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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