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Fap Polyclonal Antibody

CAC10591
Biomatik
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetFAP
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Overview

  • Supplier
    Biomatik
  • Product Name
    Fap Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC; Recommended dilution: IHC:1:20-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2191
  • Target name
    FAP
  • Target description
    fibroblast activation protein alpha
  • Target synonyms
    DPPIV, FAPA, FAPalpha, SIMP, prolyl endopeptidase FAP, 170 kDa melanoma membrane-bound gelatinase, dipeptidyl peptidase FAP, gelatine degradation protease FAP, integral membrane serine protease, post-proline cleaving enzyme, seprase, surface-expressed protease
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12884
  • Protein Name
    Prolyl endopeptidase FAP
  • Scientific Description
    The Fap Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, IHC.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Lane 1: Human U87MG cell lysates; Lane 2: Human Siha cell lysates; Lane 3: Human Hela cell lysates; Lane 4: Human A549 cell lysates probed with FAPA Polyclonal Antibody, Unconjugated (bs-5758R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of Fibroblast activation protein, alpha/FAP using anti-Fibroblast activation protein, alpha/FAP antibody (A00422-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fibroblast activation protein, alpha/FAP antigen affinity purified polyclonal antibody (Catalog # A00422-5) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fibroblast activation protein, alpha/FAP at approximately 97 kDa. The expected band size for Fibroblast activation protein, alpha/FAP is at 95 kDa.
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