Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with FAT Polyclonal Antibody, Unconjugated (bs-4278R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with FAT Polyclonal Antibody, Unconjugated (bs-4278R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
FAT Polyclonal Antibody
BS-4278R
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rat
TargetFAT1
Overview
- SupplierBioss
- Product NameFAT Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID2195
- Target nameFAT1
- Target descriptionFAT atypical cadherin 1
- Target synonymsCDHF7, CDHR8, FAT, ME5, hFat1, protocadherin Fat 1, FAT tumor suppressor 1, cadherin ME5, cadherin family member 7, cadherin-related family member 8, cadherin-related tumor suppressor homolog, protein fat homolog
- HostRabbit
- IsotypeIgG
- ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rat
- Storage Instruction-20°C
- UNSPSC12352203
Datasheet
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