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ProductsBack/FBXO5 / EMI1 Antibody (aa192-221, Biotin)

FBXO5 / EMI1 Antibody (aa192-221, Biotin)

LS-C235244
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetFBXO5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    FBXO5 / EMI1 Antibody (aa192-221, Biotin)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Biotin
  • Estimated Purity
    ...
  • Gene ID26271
  • Target name
    FBXO5
  • Target description
    F-box protein 5
  • Target synonyms
    early mitotic inhibitor 1; EMI1; F-box only protein 5; F-box protein Fbx5; FBX5; Fbxo31
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Emi1 using anti-Emi1 antibody (M05229-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Emi1 antigen affinity purified monoclonal antibody (Catalog # M05229-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Emi1 at approximately 65 kDa. The expected band size for Emi1 is at 50 kDa.
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