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WB analysis of human ferritin protein using GTX27332 Ferritin antibody. Loading : 50 ng Dilution : 1:1000
WB analysis of human ferritin protein using GTX27332 Ferritin antibody. Loading : 50 ng Dilution : 1:1000
WB analysis of human ferritin protein using GTX27332 Ferritin antibody. Loading : 50 ng Dilution : 1:1000

Ferritin antibody

GTX27332
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, Other Application
DownloadDatasheet
Product group Antibodies
TargetFTL
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Overview

  • Supplier
    GeneTex
  • Product Name
    Ferritin antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:2000-1:5000. ELISA: 1:5000-1:20000. IHC: 1:200-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, Other Application
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    10 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2512
  • Target name
    FTL
  • Target description
    ferritin light chain
  • Target synonyms
    epididymis secretory sperm binding protein; ferritin L subunit; ferritin L-chain; ferritin light chain; ferritin light polypeptide-like 3; ferritin, light polypeptide; LFTD; NBIA3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02792
  • Protein Name
    Ferritin light chain
  • Scientific Description
    This polyclonal reacts with ferritin isolated from human spleen. Ferritin is a main iron storage protein. The antibody may facilitate the analysis of ferritin in serum for an iron nutritional determination. Low values of iron are associated with iron deficiency, while high levels may indicate a malignant disease, tissue damage and other iron overload.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Ferritin Light Chain using anti-Ferritin Light Chain antibody (M01956-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ferritin Light Chain antigen affinity purified monoclonal antibody (Catalog # M01956-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ferritin Light Chain at approximately 20 kDa. The expected band size for Ferritin Light Chain is at 20 kDa.
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