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Western Blot analysis of HuvEc cells using Fes Polyclonal Antibody
Western Blot analysis of HuvEc cells using Fes Polyclonal Antibody
Western Blot analysis of HuvEc cells using Fes Polyclonal Antibody

FES Antibody

CSB-PA002518
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetFES
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Overview

  • Supplier
    Cusabio
  • Product Name
    FES Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2242
  • Target name
    FES
  • Target description
    FES proto-oncogene, tyrosine kinase
  • Target synonyms
    FPS, tyrosine-protein kinase Fes/Fps, Oncogene FES, feline sarcoma virus, feline sarcoma (Snyder-Theilen) viral (v-fes)/Fujinami avian sarcoma (PRCII) viral (v-fps) oncogene homolog, feline sarcoma oncogene, feline sarcoma/Fujinami avian sarcoma oncogene homolog, p93c-fes, proto-oncogene c-Fes, proto-oncogene c-Fps, proto-oncogene tyrosine-protein kinase Fes/Fps
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07332
  • Protein Name
    Tyrosine-protein kinase Fes/Fps
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FES using anti-FES antibody (A01453-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FES antigen affinity purified polyclonal antibody (Catalog # A01453-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FES at approximately 93 kDa. The expected band size for FES is at 93 kDa.
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