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ProductsBack/Fetuin A antibody [4N42]

Fetuin A antibody [4N42]

GTX53322
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityMouse
TargetAhsg
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Overview

  • Supplier
    GeneTex
  • Product Name
    Fetuin A antibody [4N42]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:250 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4N42
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID11625
  • Target name
    Ahsg
  • Target description
    alpha-2-HS-glycoprotein
  • Target synonyms
    alpha-2-HS-glycoprotein, countertrypin, fetuin-A
  • Host
    Rat
  • Isotype
    IgG2
  • Protein IDP29699
  • Protein Name
    Alpha-2-HS-glycoprotein
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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Figure 1. Western blot analysis of Fetuin A/AHSG using anti-Fetuin A/AHSG antibody (PB9242). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse plasma lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fetuin A/AHSG antigen affinity purified polyclonal antibody (Catalog # PB9242) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fetuin A/AHSG at approximately 55-60 kDa. The expected band size for Fetuin A/AHSG is at 39 kDa.
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