Paraformaldehyde-fixed, paraffin embedded mouse skin; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with FGF18 Polyclonal Antibody (bs-9762R) at 1:400 overnight at 4°C, followed by conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.