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WB analysis of various samples using GTX11704 FGF22 antibody. Lane 1 : rat ovary tissue lysate Lane 2 : rat ovary tissue lysate Lane 3 : rat testis tissue lysate Lane 4 : rat testis tissue lysate
WB analysis of various samples using GTX11704 FGF22 antibody. Lane 1 : rat ovary tissue lysate Lane 2 : rat ovary tissue lysate Lane 3 : rat testis tissue lysate Lane 4 : rat testis tissue lysate
WB analysis of various samples using GTX11704 FGF22 antibody. Lane 1 : rat ovary tissue lysate Lane 2 : rat ovary tissue lysate Lane 3 : rat testis tissue lysate Lane 4 : rat testis tissue lysate

FGF22 antibody

GTX11704
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetFGF22
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Overview

  • Supplier
    GeneTex
  • Product Name
    FGF22 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1-0.5microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID27006
  • Target name
    FGF22
  • Target description
    fibroblast growth factor 22
  • Target synonyms
    FGF-22; fibroblast growth factor 22
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HCT0
  • Protein Name
    Fibroblast growth factor 22
  • Scientific Description
    The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The mouse homolog of this gene was found to be preferentially expressed in the inner root sheath of the hair follicle, which suggested a role in hair development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FGF22 using anti-FGF22 antibody (PA1455). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human PC-3 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF22 antigen affinity purified polyclonal antibody (Catalog # PA1455) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF22 at approximately 24KD. The expected band size for FGF22 is at 20KD.
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