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Western blot All lanes: Fgf23 antibody at 2microg/ml + Rat heart tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 28 kDa Observed band size: 28 kDa
Western blot All lanes: Fgf23 antibody at 2microg/ml + Rat heart tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 28 kDa Observed band size: 28 kDa
Western blot All lanes: Fgf23 antibody at 2microg/ml + Rat heart tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 28 kDa Observed band size: 28 kDa

Fgf23 Antibody

CSB-PA008629LA01RA
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityRat
TargetFgf23
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Overview

  • Supplier
    Cusabio
  • Product Name
    Fgf23 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID170583
  • Target name
    Fgf23
  • Target description
    fibroblast growth factor 23
  • Target synonyms
    FGF-23; Fgf8b; fibroblast growth factor 23; fibroblast growth factor 8b
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8VI82
  • Protein Name
    Fibroblast growth factor 23
  • Scientific Description
    Regulator of phosphate homeostasis.Inhibits renal tubular phosphate transport by reducing SLC34A1 levels.Regulator of vitamin-D metabolism .Negatively regulates osteoblasts differentiation and .matrix mineralization.Acts directly on the parathyroid to decrease PTH secretion. Upregulates EGR1 .expression in the presence of KL.
  • Reactivity
    Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FGF23 using anti-FGF23 antibody (A00478-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysate, Lane 2: rat PC-12 whole cell lysate, Lane 3: mouse testis tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF23 antigen affinity purified polyclonal antibody (Catalog # A00478-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF23 at approximately 35KD. The expected band size for FGF23 is at 28KD.
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