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Western Blot analysis of SW480 cells using FGF-9 Polyclonal Antibody
Western Blot analysis of SW480 cells using FGF-9 Polyclonal Antibody
Western Blot analysis of SW480 cells using FGF-9 Polyclonal Antibody

FGF9 Antibody

CSB-PA005068
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFGF9
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Overview

  • Supplier
    Cusabio
  • Product Name
    FGF9 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2254
  • Target name
    FGF9
  • Target description
    fibroblast growth factor 9
  • Target synonyms
    FGF-9, GAF, HBFG-9, HBGF-9, SYNS3, fibroblast growth factor 9, fibroblast growth factor 9 (glia-activating factor), heparin-binding growth factor 9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31371
  • Protein Name
    Fibroblast growth factor 9
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FGF9 using anti-FGF9 antibody (A02274). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF9 antigen affinity purified polyclonal antibody (Catalog # A02274) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF9 at approximately 26KD. The expected band size for FGF9 is at 23KD.
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