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Western Blot analysis of 293 cells using Flg Polyclonal Antibody
Western Blot analysis of 293 cells using Flg Polyclonal Antibody
Western Blot analysis of 293 cells using Flg Polyclonal Antibody

FGFR1 Antibody

CSB-PA002537
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFGFR1
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Overview

  • Supplier
    Cusabio
  • Product Name
    FGFR1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2260
  • Target name
    FGFR1
  • Target description
    fibroblast growth factor receptor 1
  • Target synonyms
    basic fibroblast growth factor receptor 1; BFGFR; bFGF-R-1; CD331; CEK; ECCL; FGFBR; FGFR-1; FGFR1/PLAG1 fusion; fibroblast growth factor receptor 1; FLG; FLT2; FLT-2; FMS-like tyrosine kinase 2; fms-related tyrosine kinase 2; HBGFR; heparin-binding growth factor receptor; HH2; HRTFDS; hydroxyaryl-protein kinase; KAL2; N-SAM; OGD; proto-oncogene c-Fgr
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11362
  • Protein Name
    Fibroblast growth factor receptor 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FGFR1 using anti-FGFR1 antibody (A00098). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGFR1 antigen affinity purified polyclonal antibody (Catalog # A00098) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGFR1 at approximately 100KD. The expected band size for FGFR1 is at 91KD.
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