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FGFR2 Antibody (YA439, Rabbit)

HY-P80397
MedChem Express
TargetFGFR2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    FGFR2 Antibody (YA439, Rabbit)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID2263
  • Target name
    FGFR2
  • Target description
    fibroblast growth factor receptor 2
  • Target synonyms
    BBDS, BEK, BFR-1, CD332, CEK3, CFD1, ECT1, JWS, K-SAM, KGFR, TK14, TK25, fibroblast growth factor receptor 2, BEK fibroblast growth factor receptor, bacteria-expressed kinase, keratinocyte growth factor receptor, protein tyrosine kinase, receptor like 14
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    FGFR2 Antibody (YA439) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FGFR2.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FGFR2 using anti-FGFR2 antibody (A00231-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGFR2 antigen affinity purified polyclonal antibody (Catalog # A00231-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGFR2 at approximately 145 kDa. The expected band size for FGFR2 is at 92 kDa.
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