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FGR Polyclonal Antibody

BS-13157R
Bioss
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat
TargetFGR
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Overview

  • Supplier
    Bioss
  • Product Name
    FGR Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2268
  • Target name
    FGR
  • Target description
    FGR proto-oncogene, Src family tyrosine kinase
  • Target synonyms
    SRC2, c-fgr, c-src2, p55-Fgr, p55c-fgr, p58-Fgr, p58c-fgr, tyrosine-protein kinase Fgr, Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog, c-fgr protooncogene, c-src-2 proto-oncogene, feline Gardner-Rasheed sarcoma viral oncogene homolog, p55-c-fgr protein, proto-oncogene c-Fgr, proto-oncogene tyrosine-protein kinase FGR, v-fgr feline Gardner-Rasheed sarcoma viral oncogene homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of FGR using anti-FGR antibody (A01674-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW579 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGR antigen affinity purified polyclonal antibody (Catalog # A01674-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGR at approximately 59KD. The expected band size for FGR is at 59KD.
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