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FH antibody

10R-4123
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    FH antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Lane 1: Mouse Heart tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Rat Heart tissue lysates; Lane 4: Rat Cerebrum tissue lysates probed with FH Polyclonal Antibody, Unconjugated (bs-3938R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of FH using anti-FH antibody (A02097). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lymphaden tissue lysates, Lane 2: rat small intestine tissue lysates, Lane 3: rat gaster tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse gaster tissue lysates, Lane 7: human K562 whole cell lysates, Lane 8: human U937 whole cell lysates, Lane 9:human HL-60 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FH antigen affinity purified polyclonal antibody (Catalog # A02097) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FH at approximately 49KD. The expected band size for FH is at 55KD.
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