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ProductsBack/FH Mouse Monoclonal Antibody [Clone ID: OTI2A2]
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HeLa cells (WT) and FH-Knockdown HeLa cells (KD) were separated by SDS-PAGE and immunoblotted with anti-FH monoclonal antibody TA500681 (1:2500). Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HeLa cells (WT) and FH-Knockdown HeLa cells (KD) were separated by SDS-PAGE and immunoblotted with anti-FH monoclonal antibody TA500681 (1:2500). Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HeLa cells (WT) and FH-Knockdown HeLa cells (KD) were separated by SDS-PAGE and immunoblotted with anti-FH monoclonal antibody TA500681 (1:2500). Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.

FH Mouse Monoclonal Antibody [Clone ID: OTI2A2]

CF500681
OriGene
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityCanine, Human, Mouse, Rat
TargetFH
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    FH Mouse Monoclonal Antibody [Clone ID: OTI2A2]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2A2
  • Gene ID2271
  • Target name
    FH
  • Target description
    fumarate hydratase
  • Target synonyms
    FMRD, HLRCC, HsFH, LRCC, MCL, MCUL1, fumarate hydratase, mitochondrial, epididymis secretory sperm binding protein, fumarase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP07954
  • Protein Name
    Fumarate hydratase, mitochondrial
  • Scientific Description
    Carrier-free (BSA/glycerol-free) FH mouse monoclonal antibody, clone OTI2A2 (formerly 2A2)
  • Reactivity
    Canine, Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of FH using anti-FH antibody (A02097). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lymphaden tissue lysates, Lane 2: rat small intestine tissue lysates, Lane 3: rat gaster tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse gaster tissue lysates, Lane 7: human K562 whole cell lysates, Lane 8: human U937 whole cell lysates, Lane 9:human HL-60 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FH antigen affinity purified polyclonal antibody (Catalog # A02097) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FH at approximately 49KD. The expected band size for FH is at 55KD.
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