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ProductsBack/FHL1 Mouse Monoclonal Antibody [Clone ID: OTI2E11]
Figure A, Western blot analysis of overexpressed lysates (25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human FHL1 plasmid (RC203478, hFHL1), mouse FHL1 plasmid (MR216746, mFHL1) using anti-FHL1 antibody TA501279 (1:5000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:5000@1mg/ml).
Figure A, Western blot analysis of overexpressed lysates (25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human FHL1 plasmid (RC203478, hFHL1), mouse FHL1 plasmid (MR216746, mFHL1) using anti-FHL1 antibody TA501279 (1:5000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:5000@1mg/ml).
Figure A, Western blot analysis of overexpressed lysates (25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human FHL1 plasmid (RC203478, hFHL1), mouse FHL1 plasmid (MR216746, mFHL1) using anti-FHL1 antibody TA501279 (1:5000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:5000@1mg/ml).

FHL1 Mouse Monoclonal Antibody [Clone ID: OTI2E11]

CF501279
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityCanine, Human, Mouse, Rat
TargetFHL1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    FHL1 Mouse Monoclonal Antibody [Clone ID: OTI2E11]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2E11
  • Gene ID2273
  • Target name
    FHL1
  • Target description
    four and a half LIM domains 1
  • Target synonyms
    FCMSU, FHL-1, FHL1A, FHL1B, FLH1A, KYOT, RBMX1A, RBMX1B, SLIM, SLIM-1, SLIM1, SLIMMER, XMPMA, four and a half LIM domains protein 1, LIM protein SLIMMER, four-and-a-half Lin11, Isl-1 and Mec-3 domains 1, skeletal muscle LIM-protein 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ13642
  • Protein Name
    Four and a half LIM domains protein 1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) FHL1 mouse monoclonal antibody, clone OTI2E11 (formerly 2E11)
  • Reactivity
    Canine, Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of FHL using anti-FHL antibody (A01258-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FHL antigen affinity purified polyclonal antibody (Catalog # A01258-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FHL at approximately 32KD. The expected band size for FHL is at 36KD.
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