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ProductsBack/FHL1 Mouse Monoclonal Antibody [Clone ID: OTI3G7]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY FHL1 (Cat# RC203478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FHL1(Cat# TA501296). Positive lysates [LY400563] (100ug) and [LC400563] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY FHL1 (Cat# RC203478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FHL1(Cat# TA501296). Positive lysates [LY400563] (100ug) and [LC400563] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY FHL1 (Cat# RC203478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FHL1(Cat# TA501296). Positive lysates [LY400563] (100ug) and [LC400563] (20ug) can be purchased separately from OriGene.

FHL1 Mouse Monoclonal Antibody [Clone ID: OTI3G7]

CF501296
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFHL1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    FHL1 Mouse Monoclonal Antibody [Clone ID: OTI3G7]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI3G7
  • Gene ID2273
  • Target name
    FHL1
  • Target description
    four and a half LIM domains 1
  • Target synonyms
    FCMSU, FHL-1, FHL1A, FHL1B, FLH1A, KYOT, RBMX1A, RBMX1B, SLIM, SLIM-1, SLIM1, SLIMMER, XMPMA, four and a half LIM domains protein 1, LIM protein SLIMMER, four-and-a-half Lin11, Isl-1 and Mec-3 domains 1, skeletal muscle LIM-protein 1
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ13642
  • Protein Name
    Four and a half LIM domains protein 1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) FHL1 mouse monoclonal antibody, clone OTI3G7 (formerly 3G7)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of FHL using anti-FHL antibody (A01258-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FHL antigen affinity purified polyclonal antibody (Catalog # A01258-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FHL at approximately 32KD. The expected band size for FHL is at 36KD.
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