More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/FHL1 Mouse Monoclonal Antibody [Clone ID: OTI3H3]
Figure A, Western blot analysis of overexpressed lysates (25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human FHL1 plasmid (RC203478, hFHL1), mouse FHL1 plasmid (MR216746, mFHL1) using anti-FHL1 antibody TA501375 (1:5000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:5000@1mg/ml).
Figure A, Western blot analysis of overexpressed lysates (25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human FHL1 plasmid (RC203478, hFHL1), mouse FHL1 plasmid (MR216746, mFHL1) using anti-FHL1 antibody TA501375 (1:5000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:5000@1mg/ml).
Figure A, Western blot analysis of overexpressed lysates (25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human FHL1 plasmid (RC203478, hFHL1), mouse FHL1 plasmid (MR216746, mFHL1) using anti-FHL1 antibody TA501375 (1:5000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:5000@1mg/ml).

FHL1 Mouse Monoclonal Antibody [Clone ID: OTI3H3]

CF501375
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFHL1
Price on request
100 ug
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    OriGene
  • Product Name
    FHL1 Mouse Monoclonal Antibody [Clone ID: OTI3H3]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI3H3
  • Gene ID2273
  • Target name
    FHL1
  • Target description
    four and a half LIM domains 1
  • Target synonyms
    FCMSU, FHL-1, FHL1A, FHL1B, FLH1A, KYOT, RBMX1A, RBMX1B, SLIM, SLIM-1, SLIM1, SLIMMER, XMPMA, four and a half LIM domains protein 1, LIM protein SLIMMER, four-and-a-half Lin11, Isl-1 and Mec-3 domains 1, skeletal muscle LIM-protein 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ13642
  • Protein Name
    Four and a half LIM domains protein 1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) FHL1 mouse monoclonal antibody, clone OTI3H3 (formerly 3H3)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

Download MSDS

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
FHL1 Monoclonal AntibodyCAB5018
ReactivityHuman
TargetFHL1
  • SizePrice
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: MCF7 cells, NIH/3T3 cells, Primary antibody: CSB-PA174115(FHL1 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds
Product group Antibodies
Cusabio
FHL1 AntibodyCSB-PA174115
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetFHL1
  • SizePrice
Anti-FHL1 Antibody
Product group Antibodies
Antibodies.com
Anti-FHL1 AntibodyA14777
ApplicationsWestern Blot
ReactivityHuman, Mouse
  • SizePrice
Figure 1. Western blot analysis of FHL using anti-FHL antibody (A01258-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FHL antigen affinity purified polyclonal antibody (Catalog # A01258-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FHL at approximately 32KD. The expected band size for FHL is at 36KD.
Product group Antibodies
Boster Bio
Anti-FHL FHL1 Antibody Picoband(r)A01258-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetFHL1
  • SizePrice
Product group Antibodies
References
Everest Biotech
Goat anti-Fhl1/ Slim1 (mouse)EB06507
ApplicationsWestern Blot, ELISA
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat
TargetFHL1
  • SizePrice
Immunohistochemistry analysis in human skeletal muscle and pancreas tissues using HPA001040 antibody. Corresponding FHL1 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-FHL1 AntibodyHPA001040
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetFHL1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
SLIM / FHL1 AntibodyLS-C401682
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetFHL1
  • SizePrice
Product group Antibodies
Bioss
FHL1 Recombinant Antibody, AbBy Fluor-488 ConjugatedBSM-61727R-BF488
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetFHL1
  • SizePrice
Product group Antibodies
Biomatik
Fhl1 Polyclonal AntibodyCAC08171
ApplicationsImmunoFluorescence, Western Blot, ELISA
ReactivityMouse, Rat
TargetFHL1
  • SizePrice
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with FHL1 antibody (GTX100266) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
FHL1 antibodyGTX100266
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetFHL1
  • SizePrice