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ProductsBack/FITC Anti-Human CD19(CB19)

FITC Anti-Human CD19(CB19)

ORB2648417
Biorbyt
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
TargetCD19
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Overview

  • Supplier
    Biorbyt
  • Product Name
    FITC Anti-Human CD19(CB19)
  • Delivery Days Customer
    10
  • Application Supplier Note
    Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. FC
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CB19
  • Conjugate
    FITC
  • Gene ID930
  • Target name
    CD19
  • Target description
    CD19 molecule
  • Target synonyms
    B4, CVID3, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, T-cell surface antigen Leu-12, differentiation antigen CD19
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP15391
  • Protein Name
    B-lymphocyte antigen CD19
  • Scientific Description
    FITC Anti-Human CD19(CB19)
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of CD19 using anti-CD19 antibody (A00154-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD19 antigen affinity purified polyclonal antibody (Catalog # A00154-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD19 at approximately 85KD. The expected band size for CD19 is at 61KD.
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