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Lane 1: Jurkat cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: Mouse Brain lysates; Lane 4: Rat Brain lysates probed with FKBP1A Monoclonal Antibody, Unconjugated (bsm-51753M) at 1:4000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Jurkat cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: Mouse Brain lysates; Lane 4: Rat Brain lysates probed with FKBP1A Monoclonal Antibody, Unconjugated (bsm-51753M) at 1:4000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Jurkat cell lysates; Lane 2: MCF-7 cell lysates; Lane 3: Mouse Brain lysates; Lane 4: Rat Brain lysates probed with FKBP1A Monoclonal Antibody, Unconjugated (bsm-51753M) at 1:4000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

FKBP1A Monoclonal Antibody

BSM-51753M
Bioss
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFKBP1A
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Overview

  • Supplier
    Bioss
  • Product Name
    FKBP1A Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB(WB=1:500-2000，)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    D3J6
  • Concentration
    0.5 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID2280
  • Target name
    FKBP1A
  • Target description
    FKBP prolyl isomerase 1A
  • Target synonyms
    FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE, peptidyl-prolyl cis-trans isomerase FKBP1A, 12 kDa FK506-binding protein, 12 kDa FKBP, FK506 binding protein 1A, 12kDa, FK506 binding protein12, FK506-binding protein 1, FK506-binding protein 12, FK506-binding protein 1A, FK506-binding protein, T-cell, 12-kD, FKBP12-Exip3, PPIase FKBP1A, calstabin-1, immunophilin FKBP12, protein kinase C inhibitor 2, rotamase
  • Host
    Mouse
  • Protein IDP62942
  • Protein Name
    Peptidyl-prolyl cis-trans isomerase FKBP1A
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of FKBP1A/1B using anti-FKBP1A/1B antibody (A04492-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: huamn MOLT-4 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FKBP1A/1B antigen affinity purified polyclonal antibody (Catalog # A04492-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FKBP1A/1B at approximately 15 kDa. The expected band size for FKBP1A/1B is at 12 kDa.
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