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IHC-P analysis of endometrium tissue using GTX84491 FKBP5 antibody [3E9]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50
IHC-P analysis of endometrium tissue using GTX84491 FKBP5 antibody [3E9]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50
IHC-P analysis of endometrium tissue using GTX84491 FKBP5 antibody [3E9]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50

FKBP5 antibody [3E9]

GTX84491
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetFKBP5
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Overview

  • Supplier
    GeneTex
  • Product Name
    FKBP5 antibody [3E9]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500. ICC/IF: 1:100. IHC-P: 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2289
  • Target name
    FKBP5
  • Target description
    FKBP prolyl isomerase 5
  • Target synonyms
    AIG6, FKBP51, FKBP54, P54, PPIase, Ptg-10, peptidyl-prolyl cis-trans isomerase FKBP5, 51 kDa FK506-binding protein, 51 kDa FKBP, 54 kDa progesterone receptor-associated immunophilin, FF1 antigen, FK506 binding protein 5, FKBP-51, HSP90-binding immunophilin, PPIase FKBP5, T-cell FK506-binding protein, androgen-regulated protein 6, peptidylprolyl cis-trans isomerase, rotamase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ13451
  • Protein Name
    Peptidyl-prolyl cis-trans isomerase FKBP5
  • Scientific Description
    Interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

References

  • Repeated exposure with short-term behavioral stress resolves pre-existing stress-induced depressive-like behavior in mice. Lee EH et al., 2021 Nov 18, Nat Commun
    Read this paper

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Figure 1. Western blot analysis of FKBP5 using anti-FKBP5 antibody (M04182). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FKBP5 antigen affinity purified monoclonal antibody (Catalog # M04182) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FKBP5 at approximately 51 kDa. The expected band size for FKBP5 is at 51 kDa.
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