Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 1 antibody [C3], C-term (GTX104769) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
![Flotillin 1 antibody [C3], C-term immunoprecipitates Flotillin 1 protein in IP experiments. IP samples: HCT-116 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of Flotillin 1 protein by 4 μg Flotillin 1 antibody [C3], C-term (GTX104769) 10 % SDS-PAGE The immunoprecipitated Flotillin 1 protein was detected by Flotillin 1 antibody [C3], C-term (GTX104769) diluted at 1:1000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]](https://www.genetex.com/upload/website/prouct_img/normal/GTX104769/GTX104769_40205_IP_w_23060120_696.webp "Flotillin 1 antibody [C3], C-term immunoprecipitates Flotillin 1 protein in IP experiments. IP samples: HCT-116 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of Flotillin 1 protein by 4 μg Flotillin 1 antibody [C3], C-term (GTX104769) 10 % SDS-PAGE The immunoprecipitated Flotillin 1 protein was detected by Flotillin 1 antibody [C3], C-term (GTX104769) diluted at 1:1000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 1 antibody [C3], C-term (GTX104769) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX104769/GTX104769_40205_20250221_WB_M_R_25022521_922.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 1 antibody [C3], C-term (GTX104769) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 1 antibody [C3], C-term (GTX104769) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX104769/GTX104769_40205_20250606_WB_R_25061300_965.webp "Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 1 antibody [C3], C-term (GTX104769) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 1 antibody [C3], C-term (GTX104769) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Flotillin 1 antibody [C3], C-term
GTX104769
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHamster, Human, Mouse, Rat
TargetFLOT1
Overview
- SupplierGeneTex
- Product NameFlotillin 1 antibody [C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.34 mg/ml
- ConjugateUnconjugated
- Gene ID10211
- Target nameFLOT1
- Target descriptionflotillin 1
- Target synonymsflotillin-1, integral membrane component of caveolae
- HostRabbit
- IsotypeIgG
- Protein IDO75955
- Protein NameFlotillin-1
- Scientific DescriptionCaveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined. [provided by RefSeq]
- ReactivityHamster, Human, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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