Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 1 antibody [C3], C-term (GTX104769) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
![Flotillin 1 antibody [C3], C-term immunoprecipitates Flotillin 1 protein in IP experiments. IP samples: HCT-116 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of Flotillin 1 protein by 4 μg Flotillin 1 antibody [C3], C-term (GTX104769) 10 % SDS-PAGE The immunoprecipitated Flotillin 1 protein was detected by Flotillin 1 antibody [C3], C-term (GTX104769) diluted at 1:1000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]](https://www.genetex.com/upload/website/prouct_img/normal/GTX104769/GTX104769_40205_IP_w_23060120_696.webp "Flotillin 1 antibody [C3], C-term immunoprecipitates Flotillin 1 protein in IP experiments. IP samples: HCT-116 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of Flotillin 1 protein by 4 μg Flotillin 1 antibody [C3], C-term (GTX104769) 10 % SDS-PAGE The immunoprecipitated Flotillin 1 protein was detected by Flotillin 1 antibody [C3], C-term (GTX104769) diluted at 1:1000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 1 antibody [C3], C-term (GTX104769) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX104769/GTX104769_40205_20250221_WB_M_R_25022521_922.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 1 antibody [C3], C-term (GTX104769) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 1 antibody [C3], C-term (GTX104769) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX104769/GTX104769_40205_20250606_WB_R_25061300_965.webp "Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 1 antibody [C3], C-term (GTX104769) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 1 antibody [C3], C-term (GTX104769) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Flotillin 1 antibody [C3], C-term
GTX104769
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHamster, Human, Mouse, Rat
TargetFLOT1
Overview
- SupplierGeneTex
- Product NameFlotillin 1 antibody [C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.79 mg/ml
- ConjugateUnconjugated
- Gene ID10211
- Target nameFLOT1
- Target descriptionflotillin 1
- Target synonymsflotillin-1, integral membrane component of caveolae
- HostRabbit
- IsotypeIgG
- Protein IDO75955
- Protein NameFlotillin-1
- Scientific DescriptionCaveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined. [provided by RefSeq]
- ReactivityHamster, Human, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Zhu G, Xu J, Dai H, et al. Effect of peripheral nerve block versus general anesthesia on the hemodynamics and prognosis of diabetic patients undergoing diabetic foot Surgery. Diabetol Metab Syndr. 2023,15(1):213. doi: 10.1186/s13098-023-01185-9Read this paper
- Hsu XR, Wu JE, Wu YY, et al. Exosomal long noncoding RNA MLETA1 promotes tumor progression and metastasis by regulating the miR-186-5p/EGFR and miR-497-5p/IGF1R axes in non-small cell lung cancer. J Exp Clin Cancer Res. 2023,42(1):283. doi: 10.1186/s13046-023-02859-yRead this paper
- Pérez-Rodriguez S, de Jesús Ramírez-Lira M, Wulff T, et al. Enrichment of microsomes from Chinese hamster ovary cells by subcellular fractionation for its use in proteomic analysis. PLoS One. 2020,15(8):e0237930. doi: 10.1371/journal.pone.0237930Read this paper
- Murdica V, Cermisoni GC, Zarovni N, et al. Proteomic analysis reveals the negative modulator of sperm function glycodelin as over-represented in semen exosomes isolated from asthenozoospermic patients. Hum Reprod. 2019,34(8):1416-1427. doi: 10.1093/humrep/dez114Read this paper
- Murdica V, Giacomini E, Alteri A, et al. Seminal plasma of men with severe asthenozoospermia contain exosomes that affect spermatozoa motility and capacitation. Fertil Steril. 2019,111(5):897-908.e2. doi: 10.1016/j.fertnstert.2019.01.030Read this paper
- Rivera-Serrano EE, González-López O, Das A, et al. Cellular entry and uncoating of naked and quasi-enveloped human hepatoviruses. Elife. 2019,8. doi: 10.7554/eLife.43983Read this paper
Datasheet
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