293T whole cell extracts and 293T exosome extract (2.6 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 2 antibody [C3], C-term (GTX100279) diluted at 1:250. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
![Flotillin 2 antibody [C3], C-term detects FLOT2 protein by western blot analysis. A. 50 μg rat brain lysate/extract 10% SDS-PAGE Flotillin 2 antibody [C3], C-term (GTX100279) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100279/GTX100279_39433_WB_R_brain_w_23060100_861.webp "Flotillin 2 antibody [C3], C-term detects FLOT2 protein by western blot analysis. A. 50 μg rat brain lysate/extract 10% SDS-PAGE Flotillin 2 antibody [C3], C-term (GTX100279) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Immunoprecipitation of Flotillin 2 protein from HeLa whole cell extracts using 5 μg of Flotillin 2 antibody [C3], C-term (GTX100279). Western blot analysis was performed using Flotillin 2 antibody [C3], C-term (GTX100279). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100279/GTX100279_39443_20150625_IP_w_23060100_185.webp "Immunoprecipitation of Flotillin 2 protein from HeLa whole cell extracts using 5 μg of Flotillin 2 antibody [C3], C-term (GTX100279). Western blot analysis was performed using Flotillin 2 antibody [C3], C-term (GTX100279). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.")
![Flotillin 2 antibody [C3], C-term detects Flotillin 2 protein by Western blot analysis. Mouse tissue extracts (50 μg) was separated by 10 % SDS-PAGE, and blotted with Flotillin 2 antibody [C3], C-term (GTX100279) diluted by 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX100279/GTX100279_41731_WB_M_brain_24090423_877.webp "Flotillin 2 antibody [C3], C-term detects Flotillin 2 protein by Western blot analysis. Mouse tissue extracts (50 μg) was separated by 10 % SDS-PAGE, and blotted with Flotillin 2 antibody [C3], C-term (GTX100279) diluted by 1:1000")
![Flotillin 2 antibody [C3], C-term detects Flotillin 2 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 10 % SDS-PAGE, and blotted with Flotillin 2 antibody [C3], C-term (GTX100279) diluted by 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX100279/GTX100279_41731_WB_24090423_264.webp "Flotillin 2 antibody [C3], C-term detects Flotillin 2 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 10 % SDS-PAGE, and blotted with Flotillin 2 antibody [C3], C-term (GTX100279) diluted by 1:1000")
293T whole cell extracts and 293T exosome extract (2.6 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 2 antibody [C3], C-term (GTX100279) diluted at 1:250. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Flotillin 2 antibody [C3], C-term
GTX100279
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFLOT2
Overview
- SupplierGeneTex
- Product NameFlotillin 2 antibody [C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2319
- Target nameFLOT2
- Target descriptionflotillin 2
- Target synonymsECS-1, ECS1, ESA, ESA1, M17S1, flotillin-2, Flotillin 2 (epidermal surface antigen 1), epidermal surface antigen, membrane component chromosome 17 surface marker 1, membrane component, chromosome 17, surface marker 1 (35kD protein identified by monoclonal antibody ECS-1)
- HostRabbit
- IsotypeIgG
- Protein IDQ14254
- Protein NameFlotillin-2
- Scientific DescriptionCaveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. This gene encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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