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ProductsBack/Flt3 Ligand antibody [8D10]
ICC/IF analysis of COS7 cells transiently transfected with Flt3 Ligand plasmid using GTX84489 Flt3 Ligand antibody [8D10].
ICC/IF analysis of COS7 cells transiently transfected with Flt3 Ligand plasmid using GTX84489 Flt3 Ligand antibody [8D10].
ICC/IF analysis of COS7 cells transiently transfected with Flt3 Ligand plasmid using GTX84489 Flt3 Ligand antibody [8D10].

Flt3 Ligand antibody [8D10]

GTX84489
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetFLT3LG
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Overview

  • Supplier
    GeneTex
  • Product Name
    Flt3 Ligand antibody [8D10]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    8D10
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2323
  • Target name
    FLT3LG
  • Target description
    fms related receptor tyrosine kinase 3 ligand
  • Target synonyms
    FL, FLG3L, FLT3L, IMD125, fms-related tyrosine kinase 3 ligand, flt3 ligand, fms related tyrosine kinase 3 ligand
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP49771
  • Protein Name
    Fms-related tyrosine kinase 3 ligand
  • Scientific Description
    Stimulates the proliferation of early hematopoietic cells. Synergizes well with a number of other colony stimulating factors and interleukins.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of Flt3 ligand using anti-Flt3 ligand antibody (A04987). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Flt3 ligand antigen affinity purified polyclonal antibody (Catalog # A04987) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Flt3 ligand at approximately 26KD. The expected band size for Flt3 ligand is at 26KD.
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